2009 Southern Regional Meeting Abstracts

Purpose of Study: The high-energy intermediate of glycolysis Fructose-1,6-Diphosphate (FDP) is reported to provide significant protection during ischemia and following reperfusion of ischemic organs and tissues. Based on these observations, we investigated if FDP would provide similar protection to ex-vivo stored hearts.
Methods Used: Prior to harvesting the hearts from anesthetized rats (n=42) 19 received IV bolus of FDP (450 mg/kg; 10%) and the rest (n=23) the same amount of glucose. The hearts were removed, and aorta and left atrium were attached to modified Langerdorf apparatus and perfused with Krebs-Henseleit (n=23) and 19 with same perfusate containing 0.07 mg/ml of FDP for 15 min (37°C). Then the control hearts (n=23) were flushed with cardioplegic solution (Isolite, K+ 20 mEg/L) and the rest with the same solution containing 1 mg/ml of FDP. They were respectively placed in saline or saline containing 1 mg/ml of FDP and stored for 4 hrs. at 4°C.
Summary of Results: Pre-storage HR, AoP, CBF and cardiac output (CO) were no different between the groups. However, at 1 hr and 30 min post storage, all FDP treated hearts (n=10) were working and only 4 of the 14 controls. (p<0.05). Post-storage HR, CO, aortic systolic and mean pressures were significantly higher in the FDP group (p<0.05, p<0.02, p<0.01, and p<0.01, respectively). The myocardial ATP content at 15 min post storage reperfusion in the FDP group (n=9) was higher than in the saline group (n=9) (p<0.01). Myocardial wet/dry tissue ratio of these hearts was lower in the FDP group (p<0.01).
Conclusions: FDP significantly improved the mechanical function, attenuated ATP depletion and reduced tissue water accumulation in ex-vivo stored rat hearts during the post ischemic storage reperfusion period.