2009 Southern Regional Meeting Abstracts
Session: Allergy, Immunology, and Rheumatology
Biomarkers of Atherosclerosis in Systemic Lupus Erythematosus
Ravenell RL1, Shaftman S2, Cappuccio M4, Fleury T1, Dononhue J1, Zhu H1, Spence D3, Parker T1, Ueberroth L1, Oates J1. 1Medical University of South Carolina, Charleston, SC; 2Medical University of South Carolina, Charleston, SC; 3Robarts Research Stroke Prevention and Atherosclerosis Research Centre, London, ON, Canada and 4Medical University of South Carolina, Charleston, SC.
Purpose of Study: To describe the relationship between serum biomarkers of reactive intermediate production and the extent of atherosclerosis in patients with systemic lupus erythematosus (SLE) with the ultimate goal of developing a cardiovascular risk factor model for patients with lupus.
Methods Used: 30 subjects were enrolled between the ages of 18 and 80 and met at least four of the 1997 revised ACR SLE criteria but without a history of stroke, myocardial infarction, cardiac bypass or stenting. Personal cardiac history and Framingham risk factors were reviewed, including family history of cardiovascular disease and SLE disease activity and damage. Sera collected were analyzed for cholesterol, lupus activity markers, and biomarkers of systemic reactive intermediate production- serum 3-nitrotyrosine(3NTyr), ortho-Tyr, meta-Tyr and 3-chloroTyr by high performance liquid chromatography. Urine 8-isoprostane F2 levels were measured by immunoaffinity extraction-gas chromatography-negative ion chemical ionization-mass spectrometric detection. Carotid ultrasound measurements were performed to determine plaque area in both carotids. Descriptive analysis was performed on all variables. SPSS was used to investigate the relationship of the biomarkers with plaque area and traditional risk factors using Pearson correlation for continuous variables and chi-square test for group variables. P values <0.05 were significant.
Summary of Results: Correlation was noted between the following: age and total plaque (p=0.020), waist-hip ratio and LDL/HDL ratio (p=0.034) and LDL and ortho-tyrosine (p=0.031). A trend was also noted in total plaque and 3-nitrotyrosine levels.
Conclusions: These data suggest a relationship between the variables of age, possibly nitro-tyrosine and plaque as well as between LDL/HDL ratio and ortho-tyrosine. Our study is powered for 100 patients, and continued enrollment and anlaysis should be performed to determine if a predictive model using markers of reactive intermediate production can be developed that is more predictive than standard disease risk factors.
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