2009 Southern Regional Meeting Abstracts
Session: Joint Plenary Poster Session and Reception
VARIABILITY OF INDIVIDUAL URINE PROTEINS ASSOCIATED WITH BLOOD PRESSURE AND MEDICATIONS DIFFERENCES IN DIABETIC NEPHROPATHY
Mataria M1,2, Bland A1, Janech M1,2, Lewis E1,2, Arthur J1,2. 1Medical University of South Carolina, Charleston, SC and 2Ralph H Johnson VA Medical Center, Charleston, SC.
Purpose of Study: Identifying the effect of blood pressure control and blood pressure medications on urinary proteins abundance among diabetic patients
Methods Used: Urine samples from 18 patients with diabetic nephropathy were collected and proteins were separated by 2-D gel electrophoresis. We compared the proteins to determine if differences in medication or blood pressure at the time of collection were associated with changes in the abundance of individual proteins. Comparisons of protein abundances were made between classes of antihypertensive medications and groups of high blood pressures.
Summary of Results: . Protein spots were statistically different between the Systolic (11 spots) and diastolic pressure (12 spots) groups, ACE/ARB groups (8 spots), dihydropyridine CCB groups (2 spots) and non dihydropyridine CCB groups (8 spots). We further analyzed these groups to see if the protein abundance for the statistically different proteins segregated based on the medications or based on the blood pressure values. We did not find a major association.The greatest degree of segregation occurred for spot 2501 between with and without ACE/ARB (Panel A). There was a large amount of overlap between groups. Protein abundance did not segregate continuously based on medications either. The best correlation was for spot number 3002 shown in panel B (R2 =0.43).
Conclusions: These data confirm that factors beyond those associated with renal prognosis can change the expression of specific proteins in the urine but that there is not a strong association with any individual proteins when a group of patients is studied.
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